LOCUS       40924_23049       11558 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB459 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Tanaka Y, Nakamura S, Nakagawa T.
  TITLE     Development of the tagRFP Gateway Binary Vectors
  JOURNAL   Published Only in Database (2010)
REFERENCE   2  (bases 1 to 11558)
  AUTHORS   Tanaka Y, Nakamura S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11558)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11558)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2010)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2065..2775
                     /codon_start=1
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      2800..3052
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3086..3102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3110..3126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3134..3164)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3179..3200)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3234..3486)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3879..4667)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4691..4874)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5080..5104)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5625..6413
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6662..7250
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7436..7576)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7920..8114)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8183..9253)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9685..10311)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggtg tctaagggcg aagagctgat taaggagaac
     2101 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
     2161 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     2221 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc
     2281 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
     2341 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     2401 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
     2461 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac
     2521 cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc
     2581 cacctgatct gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
     2641 atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag
     2701 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
     2761 gggcacaaac ttaattgagc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     2821 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     2881 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     2941 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     3001 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     3061 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3121 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3181 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt cccgatctag
     3241 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     3301 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     3361 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     3421 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     3481 acgatcgggg atcatccggg tctgtggcgg gaactccacg aaaatatccg aacgcagcaa
     3541 gatatcgcgg tgcatctcgg tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac
     3601 gccgggcccg atcatattgt cgctcaggat cgtggcgttg tgcttgtcgg ccgttgctgt
     3661 cgtaatgata tcggcacctt cgaccgcctg ttccgcagag atcccgtggg cgaagaactc
     3721 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
     3781 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
     3841 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     3901 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     3961 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     4021 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     4081 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcatcgcc gtcgggcatg
     4141 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     4201 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     4261 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     4321 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     4381 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     4441 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc
     4501 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     4561 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     4621 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     4681 ccagatccgg tgcagattat ttggattgag agtgaatatg agactctaat tggataccga
     4741 ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt
     4801 gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta
     4861 aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac
     4921 gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg
     4981 ctcatgatga attcagaaat aaattcagcc taattcggcg ttaattcagt acattaaaaa
     5041 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     5101 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     5161 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     5221 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     5281 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     5341 caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     5401 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     5461 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     5521 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     5581 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg gaagcggtga
     5641 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     5701 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     5761 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     5821 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     5881 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     5941 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     6001 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     6061 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     6121 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     6181 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     6241 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     6301 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     6361 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc
     6421 tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga
     6481 tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct tttattattt
     6541 ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg accaaaatcc
     6601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6661 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6721 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6781 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6841 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6901 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6961 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7021 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7081 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7141 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7201 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7261 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7321 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7381 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     7441 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     7501 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     7561 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     7621 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     7681 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     7741 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     7801 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     7861 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     7921 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     7981 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     8041 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     8101 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     8161 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     8221 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     8281 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     8341 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     8401 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     8461 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     8521 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     8581 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     8641 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     8701 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     8761 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     8821 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     8881 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     8941 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     9001 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     9061 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     9121 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     9181 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
     9241 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
     9301 gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
     9361 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
     9421 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
     9481 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
     9541 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
     9601 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
     9661 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
     9721 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
     9781 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
     9841 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
     9901 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
     9961 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
    10021 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
    10081 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
    10141 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
    10201 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    10261 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    10321 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    10381 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    10441 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    10501 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    10561 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    10621 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    10681 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    10741 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    10801 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    10861 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    10921 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    10981 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    11041 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    11101 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    11161 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    11221 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    11281 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    11341 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    11401 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    11461 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
    11521 gttggcatgc acatacaaat ggacgaacgg ataaacct
//
